Fig. 3

Effect of preincubation with anti-CXCR4 and anti-CCR3 antibody on r-Nef-dependent human basophil chemotaxis a Basophils were incubated with (grey histogram) or without (white histogram) anti-CXCR4 antibody (5 µg/ml) for 1 h, then loaded into the chemotaxis chamber and allowed to migrate toward the indicated concentrations of r-Nef, CXCL12/SDF-1α and fMLF for 1 h at 37 °C in a humidified incubator with 5% CO₂. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to control. b Basophils were incubated with (grey histogram) or without (white histogram) anti-CCR3 antibody (5 µg/ml) for 1 h, then loaded into the chemotaxis chamber and allowed to migrate toward the indicated concentrations of r-Nef, CXCL12/SDF-1α and Tat protein for 1 h at 37 °C in a humidified incubator with 5% CO₂. Values are the mean ± SEM of three distinct experiments. *p < 0.05 as compared to control